Ioannis Ragoussis Ph.D.

Ioannis Ragoussis

Ioannis Ragoussis

Ioannis (Jiannis) Ragoussis has studied Biochemistry at the University of Tuebingen, Germany, where he obtained his PhD. He did his postdoctoral studies as EMBO fellow with J. Trowsdale at the Imperial Cancer Research Fund Laboratories in London, UK. Subsequently he became Lecturer and then Senior Lecturer at the Division of Medical and Molecular Genetics at Guy’s Hospital, then Guy’s and now King’s Medical School. In 2001 he moved to the University of Oxford as Head of Genomics and in 2013 to McGill University where he is Head of Genome Sciences at the McGill University and Genome Quebec Innovation Centre (MUGQIC) and Associate Professor in Human Genetics and at the Department of Bioengineering. He is also affiliated Professor at the Biomedical Sciences research Centre A. Fleming in Greece and the King Abdulaziz University. He has developed expertise in all fields of genomics and functional genomics. His main interest is on developing NGS based approaches to disease gene identification, as well as functional genomics approaches for the identification of prognostic markers and associated pathways in breast cancer.


Research Interests

As head of Genome Sciences. he is responsible for the Genomic Platform Development within the MUGQIC Genome Canada Genome Innovation Node and Canada’s Genomics Enterprize funded by CFI. He oversees genomic platform operation and new technology evaluation and integration. His Lab works on Technical Developments including the Single Cell Genomics Lab of MUGQIC and Mutation Detection Technologies with focus on the genomic and transcriptomic analysis of single cells. Supported by CFI the lab includes a Fluidigm BioMark HD instrument and two Fluidigm C1 instruments, a 10X Genomics Chromium instrument as well as low volume liquid handling instruments. The Ragoussis lab is a member of McGill’s Integrative and Quantitative Biology Initiative, lead by Prof. Jacalyn Vogel at the Department of Biology. The lab is responsible for single molecule sequencing using PacBio Sequel technology. The single Cell Genomics laboratory is open to Investigators from McGill University and for collaborations with any other Institute, offering single cell expression profiling and DNA analysis including exome Sequencing. Both single cell whole genome expression profiling as well as whole exome sequencing protocols are validated and available for external users.

For enquiries on single cell technology, 10X Genomics and single molecule Pacbio sequencing please email: ioannis.ragoussis@mcgill.ca

Projects

1. Singele Cell Genomics: This work is in collaboration with Professors Morag Park (Tumor Heterogeneity and Microenvironment) Richard Kremer (Circulating Tumor Cells), Guillaume Bourque, Kevin Petrecca, Gustavo Turecki and others. Technical developments include single cell tagging in collaboration with Claudia Kleinman and Santiago Constantino supported by a Genome Canada Disruptive Technologies award.

2. Methodologies for the highly sensitive detection of both somatic as well as germline mutations: Collaboration with Will Foulkes (Inherited Breast Cancer, DICER1 Syndrome), Lucy Gilbert ( Endometrial Cancer) & Bert Vogelstein (Johns Hopkins) and Patricia Tonin (Inherited Breast and Ovarian Cancer).

3. The development of long read single molecule sequencing based methodologies and diagnostic tests in collaboration with Cheelong Shaw and as partner of the igeneTRAIN Consortium (Philadeplhia Childrens Hospital).

4. High Throughput Whole Genome Genotyping using Affymetrix Axiom technology is performed as part of Canada’s Longitudinal Study of Aging.

The lab collaborates with companies such as Fluidigm, Agilent, Wafergen, 10X Genomics and Oxford Nanopore with further support from Compute Canada, CFI, Department of Defense and others.

Teaching

* Graduate course HGEN 400, Genetics in Medicine

* Postgraduate cuurse HGEN 676, Laboratory Course in Genomics.

For enquiries please email: ioannis.ragoussis@mcgill.ca

Lab Composition

Yu Chang Wang Lab Manager
Timotee Revil Postdoctoral Research Associate
Charler Meunier Postdoctoral Research Associate
Dunarel Badescu Research Associate in Bioinformatics
Yoshihiko Nagai Research Associate in Bioinformatics
Spyridon Oikonomopoulos Postdoctoral Fellow
Corinne Zwaig Research Assistant
Alexandre Beslile Research Assistant
Shu-Huang Wang Research Assistant
Alexandre Mazure Research Associate in Informatics
Sommayyeh Fahiminiya Visiting Research Associate in Bioinformatics
Setor Amuzu PhD student, Queen Elizabeth II scholar
Anthony Bayega PhD student, Queen Elizabeth II scholar